MEK1 / 2 (phospho Ser218 / Ser222) Antibody
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產(chǎn)品名稱: MEK1 / 2 (phospho Ser218 / Ser222) Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
MEK1 / 2 (phospho Ser218 / Ser222) Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。MEK1 / 2 (phospho Ser218 / Ser222) Antibody嚴(yán)格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
MEK1 / 2 (phospho Ser218 / Ser222) Antibody
的詳細(xì)介紹
MEK1 / 2 (phospho Ser218 / Ser222) AntibodyProduct Profile
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Product Name
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MEK1 / 2 (phospho Ser218 / Ser222) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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MEK 1 and MEK 2 are integral components of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activation of MEK 1/2 occurs via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK. This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade.1) N.G. Ahn, N.G., Robbins, D.G., Haycock, J.W., Seger, R., Cobb, M.H. and Krebs, E.G., "Identification of an activator of the microtubule-associated protein 2 kinases ERK1 and ERK2 in PC12 cells stimulated with nerve growth factor or bradykinin," J. Neurochem. 59 (1992) 147 - 156.
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Immunogen
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MEK 1/2 (Ser218/222) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer218/222 of human MEK 1/2.
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MEK1 / 2 (phospho Ser218 / Ser222) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Tested Applications
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WB
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The antibody immunolabels the ~45k MEK protein in a Western blot of serum-stimulated NIH 3T3 cells. The immunolabeling is blocked by the phosphopeptide used as antigen but not the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.:
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Species Reactivity
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HumanMouseRatXenopus
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Concentration
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1mg/ml
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Purification
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Affinity purified
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MEK1 / 2 (phospho Ser218 / Ser222) AntibodyTarget Information
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Gene Symbol
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MAP2K1
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Alternative Names
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CFC3
MEK1
MKK1
MAPKK1
PRKMK1
ERK activator kinase 1
MAP kinase kinase 1
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Molecular Weight(MW)
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45
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Tissue Specificity
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MEK1/2 antibody is specific for MEK 1/2 phosphorylated at Ser218/222.
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MEK1 / 2 (phospho Ser218 / Ser222) AntibodyDatabase Links
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Entrez Gene
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5604
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Protein Accession
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Q02750
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MEK1 / 2 (phospho Ser218 / Ser222) AntibodyApplication
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Application
Western blot of NIH 3T3 cell lysates. The cells were serum starved (control) or incubated in the presence of serum (serum) for 5 minutes. The western blot shows the immunolabeling of the ~45k MEK1/2 was absent in control and stimulated by serum.
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Application Notes
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The antibody immunolabels the ~45k MEK protein in a Western blot of serum-stimulated NIH 3T3 cells. The immunolabeling is blocked by the phosphopeptide used as antigen but not the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.:
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Additional Information
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Form
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Liquid
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Storage Instructions
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For long term storage –80?C is recommended, but shorter term storage at –20?C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
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Storage Buffer
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100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
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