MEK1 (phospho Thr386) Antibody
如果您對該產(chǎn)品感興趣的話,可以
產(chǎn)品名稱: MEK1 (phospho Thr386) Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
MEK1 (phospho Thr386) Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。MEK1 (phospho Thr386) Antibody嚴(yán)格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
MEK1 (phospho Thr386) Antibody
的詳細(xì)介紹
MEK1 (phospho Thr386) AntibodyProduct Profile
|
Product Name
|
MEK1 (phospho Thr386) Antibody
|
|
Antibody Type
|
Primary Antibodies
|
|
Product description
|
MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386.1) Park, S.H., Zarrinpar, A. and Lim, W.A., "Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms," Science 299 (2003) 1061 - 1064.
More
|
|
Immunogen
|
MEK 1 (Thr386) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr386 of human MEK 1.
|
MEK1 (phospho Thr386) AntibodyKey Feature
|
Clonality
|
Polyclonal
|
|
Host Species
|
Rabbit
|
|
Species Reactivity
|
Human
|
|
Concentration
|
1mg/ml
|
|
Purification
|
Affinity purified
|
MEK1 (phospho Thr386) AntibodyTarget Information
|
Gene Symbol
|
MAP2K1
|
|
Alternative Names
|
CFC3
MEK1
MKK1
MAPKK1
PRKMK1
ERK activator kinase 1
MAP kinase kinase 1
More
|
|
Molecular Weight(MW)
|
45
|
|
Tissue Specificity
|
MEK1 antibody is specific for the 45k MEK 1 phosphorylated at Thr386.
|
MEK1 (phospho Thr386) AntibodyDatabase Links
|
Entrez Gene
|
5604
|
|
Protein Accession
|
Q02750
|
MEK1 (phospho Thr386) AntibodyApplication
-
Application
Western blot of human T47D cells showing phospho-specific immunolabeling of the ~45k MEK1 protein phosphorylated at Thr386.
|
Application Notes
|
Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.:
|
Additional Information
|
Form
|
Liquid
|
|
Storage Instructions
|
For long term storage –80?C is recommended, but shorter term storage at –20?C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
|
|
Storage Buffer
|
100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
|
