MEK1 (phospho Thr386) Antibody
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產品名稱: MEK1 (phospho Thr386) Antibody
產品型號:
產品展商: immunoclone
產品文檔: 無相關文檔
簡單介紹
MEK1 (phospho Thr386) Antibody迅速增長的產品線包括一抗、二抗、**測定及細胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。MEK1 (phospho Thr386) Antibody嚴格的質量控制和驗證實驗包括各種不同的應用：WB、細胞**熒光、**組化、流式細胞術、ELISA、CHIP、IP和peptide array。
MEK1 (phospho Thr386) Antibody
的詳細介紹
MEK1 (phospho Thr386) AntibodyProduct Profile
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Product Name
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MEK1 (phospho Thr386) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386.1) Park, S.H., Zarrinpar, A. and Lim, W.A., "Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms," Science 299 (2003) 1061 - 1064.
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Immunogen
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MEK 1 (Thr386) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr386 of human MEK 1.
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MEK1 (phospho Thr386) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Species Reactivity
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Human
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Concentration
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1mg/ml
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Purification
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Affinity purified
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MEK1 (phospho Thr386) AntibodyTarget Information
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Gene Symbol
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MAP2K1
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Alternative Names
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CFC3
MEK1
MKK1
MAPKK1
PRKMK1
ERK activator kinase 1
MAP kinase kinase 1
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Molecular Weight(MW)
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45
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Tissue Specificity
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MEK1 antibody is specific for the 45k MEK 1 phosphorylated at Thr386.
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MEK1 (phospho Thr386) AntibodyDatabase Links
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Entrez Gene
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5604
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Protein Accession
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Q02750
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MEK1 (phospho Thr386) AntibodyApplication
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Application
Western blot of human T47D cells showing phospho-specific immunolabeling of the ~45k MEK1 protein phosphorylated at Thr386.
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Application Notes
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Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.:
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Additional Information
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Form
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Liquid
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Storage Instructions
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For long term storage –80?C is recommended, but shorter term storage at –20?C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
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Storage Buffer
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100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
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