MEK1 (phospho Ser221) Antibody
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產(chǎn)品名稱: MEK1 (phospho Ser221) Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
MEK1 (phospho Ser221) Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。MEK1 (phospho Ser221) Antibody嚴(yán)格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
MEK1 (phospho Ser221) Antibody
的詳細(xì)介紹
MEK1 (phospho Ser221) AntibodyProduct Profile
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Product Name
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MEK1 (phospho Ser221) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.1) Zebisch A, et al. (2006) Cancer Res; 66 (7): 3401-8. Luciano BS, et al. (2006) J Biol Chem; 279 (50): 52117-23. Wang X, et al. (2003) Oncogene; 22 (1): 109-16. Gopalbhai K, et al. (2003) J Biol Chem; 278 (10): 8118-25. Ling MT, et al. (2002) Oncogene; 21 (55): 8498-505.
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Immunogen
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MEK1 (Phospho-Ser221) antibody was raised against a peptide sequence around phosphorylation site of serine 221 (A-N-S (p) -F-V) derived from Human MEK1.
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MEK1 (phospho Ser221) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Tested Applications
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IFIHCWB
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
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Species Reactivity
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HumanMouseRat
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Concentration
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1mg/ml
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Purification
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Affinity purified
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MEK1 (phospho Ser221) AntibodyTarget Information
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Gene Symbol
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MAP2K1
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Alternative Names
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CFC3
MEK1
MKK1
MAPKK1
PRKMK1
EMS1
ERK activator kinase 1
MAP kinase kinase 1
MAP2K1
MAPK/ERK kinase 1
MAPKK 1
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Molecular Weight(MW)
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45 kDa
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Tissue Specificity
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This antibody detects endogenous level of MEK1 only when phosphorylated at serine 221.
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MEK1 (phospho Ser221) AntibodyDatabase Links
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Entrez Gene
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5604
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Protein Accession
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NP_002746.1
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MEK1 (phospho Ser221) AntibodyApplication
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Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Phospho-Ser221). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MEK1 (Phospho-Ser221).
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Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using MEK1 (Phospho-Ser221).
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Application Notes
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store antibody at -20?C for up to one year.
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Storage Buffer
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Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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