IkB a (phospho Ser32 / Ser36) Antibody
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產(chǎn)品名稱: IkB a (phospho Ser32 / Ser36) Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
IkB a (phospho Ser32 / Ser36) Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動(dòng)劑/拮抗劑/活化劑/抑制劑及裂解物等。IkB a (phospho Ser32 / Ser36) Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
IkB a (phospho Ser32 / Ser36) Antibody
的詳細(xì)介紹
IkB a (phospho Ser32 / Ser36) AntibodyProduct Profile
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Product Name
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IkB a (phospho Ser32 / Ser36) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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Inhibits the activity of dimeric NF-κ-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.1) Shieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823. Honda, R. et al. (1997) FEBS Lett. 420, 25-27. Hirao, A. et al. (2000) Science 287, 1824-1827. Tibbetts, R. S. et al. (1999) Genes Dev. 13, 152-157. Chehab, N. H. et al. (1999) Proc . Natl . Acad. Sci. USA 96, 13777-13782.
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Immunogen
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IkB-a (Phospho-Ser32/Ser36) antibody was raised against a peptide sequence around phosphorylation site of serine 32/36 (H-D-S (p) -G-L-D-S (p) -M-K) derived from Human IkappaB-α.
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IkB a (phospho Ser32 / Ser36) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Tested Applications
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IFIHCWB
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
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Species Reactivity
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HumanMouseRat
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Concentration
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1mg/ml
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Purification
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Affinity purified
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IkB a (phospho Ser32 / Ser36) AntibodyTarget Information
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Gene Symbol
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NFKBIA
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Alternative Names
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IKBA
MAD-3
NFKBI
ADDA
I-kappa-B-alpha
IKBA
NF-kappaB inhibitor alpha
NFKBIA
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Molecular Weight(MW)
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39 kDa
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Tissue Specificity
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This antibody detects endogenous level of IkappaB-α only when phosphorylated at serine 32/36.
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IkB a (phospho Ser32 / Ser36) AntibodyDatabase Links
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Entrez Gene
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4792
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Protein Accession
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NP_065390.1
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IkB a (phospho Ser32 / Ser36) AntibodyApplication
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Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-α (Phospho-Ser32/Ser36). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using IkappaB-α (Phospho-Ser32/Ser36).
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Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF using IκB-α (Phospho-Ser32/Ser36).
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Application Notes
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store antibody at -20?C for up to one year.
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Storage Buffer
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Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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