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產(chǎn)品名稱: 27Kip1 Antibody
產(chǎn)品型號(hào):
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
27Kip1 Antibody迅速增長(zhǎng)的產(chǎn)品線包括一抗、二抗、**測(cè)定及細(xì)胞檢測(cè)試劑盒、蛋白/多肽、激動(dòng)劑/拮抗劑/活化劑/抑制劑及裂解物等。27Kip1 Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
27Kip1 Antibody
的詳細(xì)介紹
27Kip1 AntibodyProduct Profile
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Product Name
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27Kip1 Antibody
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Antibody Type
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Primary Antibodies
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Product description
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p27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.1) Kawamata, N., et al., Eur. J. Haematol. 74(5):424-429 (2005).
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Immunogen
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This 27Kip1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-194 amino acids from the C-terminal region of human 27Kip1.
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27Kip1 AntibodyKey Feature
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Clonality
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Polyclonal
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Isotype
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Ig
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Host Species
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Rabbit
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Tested Applications
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IFWB
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For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:
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Species Reactivity
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Human
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Concentration
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1mg/ml
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Purification
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Affinity purified
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27Kip1 AntibodyTarget Information
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Gene Symbol
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CDKN1B
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Alternative Names
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Cyclin-dependent kinase inhibitor 1B
Cyclin-dependent kinase inhibitor p27
p27Kip1
CDKN1B
KIP1
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Molecular Weight(MW)
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22 kDa
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Sequence Similarities
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Predicted species reactivity based on immunogen sequence: Hamster
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27Kip1 AntibodyDatabase Links
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Entrez Gene
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1027
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Protein Accession
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P46527
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27Kip1 AntibodyApplication
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Application
Western Blot in HepG2 cell line lysates (35ug/lane). 27Kip1(arrow) was detected using the purified Pab.
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Application
Confocal immunofluorescent analysis of 27Kip1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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Application Notes
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For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Storage Buffer
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Supplied in PBS with 0.09% (W/V) sodium azide.
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