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產(chǎn)品資料

RPS6K1 Antibody

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產(chǎn)品名稱: RPS6K1 Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

RPS6K1 Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。RPS6K1 Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用:WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。


RPS6K1 Antibody  的詳細(xì)介紹

RPS6K1 AntibodyProduct Profile

Product Name RPS6K1 Antibody
Antibody Type Primary Antibodies
Product description
					
RPS6K1 Antibody: Ribosomal protein S6 kinase 1 (RPS6K1) is the best characterized effector of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Nutrients and growth factors stimulate a complex including TOR, raptor (regulatory associated protein of TOR), and GbetaL to phosphorylate RPS6K1 and the eukaryotic initiation factor 4E binding protein (4EBP1), leading to increased protein synthesis and cell growth. RPS6K1 is thought to desensitize tissues to insulin as mice deficient in RPS6K1 have been shown to be hypersensitive to insulin and impervious to obesity-induced insulin resistance seen in wild type obese mice.1) Burnett PE, Barrow RK, Cohen NA, et al. RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1. Proc. Natl. Acad. Sci. USA. 1998; 95:1432-7.
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Immunogen
RPS6K1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human RPS6K1. The immunogen is located within amino acids 600 - 650 of RPS6K1.

RPS6K1 AntibodyKey Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAICCIFWB

RPS6K1antibody can be used for the detection of RPS6K1 by Western blot at 2.5 - 10 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

RPS6K1 AntibodyTarget Information

Gene Symbol RPS6KA1
Alternative Names
RPS6K1 Antibody: RSK
HU-1
RSK1
MAPKAPK1A
Ribosomal protein S6 kinase alpha-1
90 kDa ribosomal protein S6 kinase 1
S6K-alpha-1
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Sequence Similarities 2) Cat. No. 17-005 - Jurkat Cell Slide

RPS6K1 AntibodyDatabase Links

Entrez Gene 6195
Protein Accession AAC82497

RPS6K1 AntibodyApplication

  • RPS6K1 Antibody
    Application

    Immunofluorescence of RP S6 Kinase in Jurkat cells with RP S6 Kinase antibody at 20 μg/mL.

  • RPS6K1 Antibody
    Application

    Immunocytochemistry of RPS6K1 in Jurkat cells with RPS6K1 antibody at 10 μg/mL.

  • RPS6K1 Antibody
    Application

    Western blot analysis of RPS6K1 in Jurkat cell lysate with RPS6K1 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.

Positive Control 1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
RPS6K1antibody can be used for the detection of RPS6K1 by Western blot at 2.5 - 10 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.:

Additional Information

Form Liquid
Storage Instructions RPS6K1 antibody can be stored at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer RPS6K1 Antibody is supplied in PBS containing 0.02% sodium azide.

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