Product Name | Cyclin D1 Antibody |
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Antibody Type | Primary Antibodies |
Product description |
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Immunogen |
This Cyclin D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from human Cyclin D1. |
Clonality | Polyclonal |
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Isotype | Ig |
Host Species | Rabbit |
Tested Applications | |
For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:2000 For IF starting dilution is: 1:10~50:
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Species Reactivity | |
Concentration | 1mg/ml |
Purification | Affinity purified |
Gene Symbol | CCND1 |
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Alternative Names |
G1/S-specific cyclin-D1
More
B-cell lymphoma 1 protein BCL-1 BCL-1 oncogene PRAD1 oncogene CCND1 BCL1 PRAD1 |
Molecular Weight(MW) | 34 kDa |
Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Entrez Gene | 595 |
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Protein Accession | P24385 |
Confocal immunofluorescent analysis of Cyclin D1 Antibody (S90) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Western blot analysis of (S90) in mouse lung lysates (35ug/lane).
Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: A431 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Antibody staining Cyclin D1 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application Notes |
For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:2000 For IF starting dilution is: 1:10~50:
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Form | Liquid |
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Storage Instructions | Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |