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產(chǎn)品名稱: CCND1-Y226 Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
CCND1-Y226 Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。CCND1-Y226 Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
CCND1-Y226 Antibody
的詳細(xì)介紹
CCND1-Y226 AntibodyProduct Profile
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Product Name
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CCND1-Y226 Antibody
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Antibody Type
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Primary Antibodies
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Product description
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CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.1) Edel, M.J., et al. Genes Dev. 24(6):561-573(2010)
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Immunogen
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This CCND1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-238 amino acids from human CCND1.
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CCND1-Y226 AntibodyKey Feature
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Clonality
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Polyclonal
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Isotype
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Ig
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Host Species
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Rabbit
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Tested Applications
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IFWB
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For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:
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Species Reactivity
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Human
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Concentration
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1mg/ml
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Purification
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Affinity purified
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CCND1-Y226 AntibodyTarget Information
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Gene Symbol
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CCND1
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Alternative Names
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G1/S-specific cyclin-D1
B-cell lymphoma 1 protein
BCL-1
BCL-1 oncogene
PRAD1 oncogene
CCND1
BCL1
PRAD1
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Molecular Weight(MW)
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34 kDa
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CCND1-Y226 AntibodyDatabase Links
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Entrez Gene
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595
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Protein Accession
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P24385
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CCND1-Y226 AntibodyApplication
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Application
Confocal immunofluorescent analysis of CCND1-Y226 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstratesdetected the CCND1 protein (arrow).
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Application Notes
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For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Storage Buffer
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Supplied in PBS with 0.09% (W/V) sodium azide.
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