MEK 2 (phospho Thr394) Antibody
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產(chǎn)品名稱: MEK 2 (phospho Thr394) Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
MEK 2 (phospho Thr394) Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。MEK 2 (phospho Thr394) Antibody嚴格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用：WB、細胞**熒光、**組化、流式細胞術(shù)、ELISA、CHIP、IP和peptide array。
MEK 2 (phospho Thr394) Antibody
的詳細介紹
MEK 2 (phospho Thr394) AntibodyProduct Profile
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Product Name
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MEK 2 (phospho Thr394) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.1) Crews C M, et al. (1992) Science. 258:478-480. Alessi D R, et al. (1994) EMBO J. 13:1610-1619. Rosen L B, et al. (1994) Neuron. 12:1207-1221. Cowley S, et al. (1994) Cell. 77:841-852.
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Immunogen
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MEK-2 (Phospho-Thr394) antibody was raised against a peptide sequence around phosphorylation site of threonine 394 (P-G-T (p) -P-T) derived from Human MEK-2.
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MEK 2 (phospho Thr394) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Tested Applications
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IFIHCWB
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
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Species Reactivity
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HumanMouseRat
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Concentration
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1mg/ml
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Purification
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Affinity purified
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MEK 2 (phospho Thr394) AntibodyTarget Information
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Gene Symbol
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MAP2K2
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Alternative Names
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CFC4
MEK2
MKK2
MAPKK2
PRKMK2
SRC1
ERK activator kinase 2
MAP kinase kinase 2
MAP2K2
MAPK/ERK kinase 2
MAPKK 2
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Molecular Weight(MW)
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44 kDa
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Tissue Specificity
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This antibody detects endogenous level of MEK2 only when phosphorylated at threonine 394.
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MEK 2 (phospho Thr394) AntibodyDatabase Links
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Entrez Gene
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5605
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Protein Accession
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NP_109587.1
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MEK 2 (phospho Thr394) AntibodyApplication
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Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2 (Phospho-Thr394). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MEK-2 (Phospho-Thr394).
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Application
Western blot analysis of lysed extracts from HepG2 and HeLa cells untreated or treated with UV using MEK-2 (Phospho-Thr394).
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Application Notes
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store antibody at -20?C for up to one year.
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Storage Buffer
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Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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