Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This protein is the major AP endonuclease in human cells.1) Vascotto,C., Mol. Cell. Biol. 29 (7), 1834-1854 (2009) More
This APEX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-64 amino acids from the N-terminal region of human APEX1.
IFWB
Human
Confocal immunofluorescent analysis of APEX1 Antibody with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of anti-APEX1 Antibody in Hela cell line lysates (35ug/lane)
Western blot analysis of APEX1 using rabbit polyclonal APEX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APEX1 gene.
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