HEC-1-A細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

組織來(lái)源： endometrium

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

器官來(lái)源： **

細(xì)胞形態(tài)： 上皮樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

年限： 71 years

ATCC Number： HTB-112?

相關(guān)**： 腺癌

Designations: HEC-1-A

HEC-1-A細(xì)胞Depositors: H Kuramoto

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: uterus

Tissue: endometrium

Disease: adenocarcinoma

Cellular Products: platelet activating factor (PAF)

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Receptors: HEC-1-A細(xì)胞platelet activating factor (PAF)

Tumorigenic: Yes

Oncogene: c-fos +

Antigen Expression: Blood Type B; Rh+

Cytogenetic Analysis: hypodiploid to hyperdiploid, modal number = 47 with large metacentric marker

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1

PGM3, 1-2

Age: 71 years

Gender: female

Comments: HEC-1-A細(xì)胞This line and a substrain HEC-1-B (ATCC HTB-113) were isolated in 1968 by H. Kuramoto and associates from a patient with stage IA endometrial cancer.

PAF induces increased expression of c-fos.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007

recommended serum:ATCC 30-2020

References: 22449: HEC-1-A細(xì)胞Kuramoto H. Studies of the growth and cytogenetic properties of human endometrial adenocarcinoma in culture and its development into an established line. Acta Obstet. Gynaecol. Jpn. 19: 47-58, 1972. PubMed: 4678779

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

23069: Presta M, et al. Modulation of plasminogen activator activity in human endometrial adenocarcinoma cells by basic fibroblast growth factor and transforming growth factor beta. Cancer Res. 48: 6384-6389, 1988. PubMed: 3263185

23115: Maggi M, et al. Platelet-activating factor mediates an autocrine proliferative loop in the endometrial adenocarcinoma cell line HEC-1A. Cancer Res. 54: 4777-4784, 1994. PubMed: 7520361

23541: Kuramoto H, et al. Establishment of a cell line of human endometrial adenocarcinoma in vitro. Am. J. Obstet. Gynecol. 114: 1012-1019, 1972. PubMed: 4673779

29988: Hendricks DT, et al. HEC-1-A細(xì)胞FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105