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NCI-H929細(xì)胞

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產(chǎn)品名稱: NCI-H929細(xì)胞
產(chǎn)品型號(hào): NCI-H929
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡(jiǎn)單介紹

NCI-H929細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。NCI-H929細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長(zhǎng)密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


NCI-H929細(xì)胞  的詳細(xì)介紹

NCI-H929細(xì)胞

ATCC Number: CRL-9068?

數(shù)量: 大量

相關(guān)**: 多發(fā)性骨髓瘤

細(xì)胞類型: B**細(xì)胞

是否是腫瘤細(xì)胞: 1

物種來源: 人

運(yùn)輸方式: 凍存運(yùn)輸

細(xì)胞形態(tài): **樣

**類型: IgA kappa (Recent tests for IgA, kappa have not detected the production of IgA; the cells are producing kappa light chain.)

年限: 62 years

器官來源: 骨髓

生長(zhǎng)狀態(tài): 懸浮生長(zhǎng)

Designations: NCI-H929 [H929]

NCI-H929細(xì)胞Depositors: National Cancer Institute

Isotype: IgA kappa (Recent tests for IgA, kappa have not detected the production of IgA; the cells are producing kappa light chain.)

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast


Source: Organ: bone marrow

Disease: plasmacytoma; myeloma

Cell Type: B lymphocyte;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors: transferrin

Antigen Expression: PCA-1; CD38 +

DNA Profile (STR): NCI-H929細(xì)胞Amelogenin: X

CSF1PO: 11

D13S317: 12

D16S539: 9,13

D5S818: 11,12

D7S820: 10,12

THO1: 9.3

TPOX: 8,11

vWA: 14,15

Cytogenetic Analysis: Near tetraploid. Most copies of chromosome 8 have the 8q+ abnormality.

Age: 62 years

Gender: female

Ethnicity: Caucasian

Comments: This cell line was established from a malignant effusion in a patient with myeloma.

The cells are positive for plasma cell antigen 1 (PCA-1), transferrin receptor, CD38 (T10), but are negative for HLA DR, CALLA and markers of early B cell development.

NCI-H929細(xì)胞They are negative for Epstein-Barr virus nuclear antigen (EBNA).

The cells have a rearrangement of the c-myc proto oncogene and express c-myc RNA.

There is also an activated ras allele.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 4 X 10(5) viable cells/ml.

Interval: Maintain cultures at cell concentrations between 5 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22372: Hollis GF, et al. NCI-H929細(xì)胞Complex translocation disrupts c-myc regulation in a human plasma cell myeloma. Mol. Cell. Biol. 8: 124-129, 1988. PubMed: 3275865

23349: Gazdar AF, et al. Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene. Blood 67: 1542-1549, 1986. PubMed: 2423157

23351: Ernst TJ, et al. Identification of a second transforming gene, rasn, in a human multiple myeloma line with a rearranged c-myc allele. Blood 72: 1163-1167, 1988. PubMed: 3048435

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