Hs 737.T細胞

運輸方式： 凍存運輸

年限： 10 years

生長狀態(tài)： 貼壁生長

ATCC Number： CRL-7473?

數(shù)量： 大量

相關(guān)**： 巨細胞肉瘤

是否是腫瘤細胞： 1

物種來源： 人

器官來源： 骨

細胞形態(tài)： 成纖維樣

Designations: Hs 737.T

Hs 737.T細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: bone

Disease: giant cell sarcoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

DNA Profile (STR): Hs 737.T細胞Amelogenin: X

CSF1PO: 10

D13S317: 11

D16S539: 9,11

D5S818: 9,11

D7S820: 8,12

THO1: 6,9

TPOX: 8,9

vWA: 16,18

Cytogenetic Analysis: modal number = 46; range = 45 to 46

Age: 10 years

Gender: female

Ethnicity: Caucasian

Comments: Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Please see the NBL Repository description.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Hs 737.T細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to the flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Subcultivation Ratio: 1:2 to 1:3

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Hs 737.T細胞culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase