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產(chǎn)品資料

Hs 343.T細(xì)胞

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產(chǎn)品名稱: Hs 343.T細(xì)胞
產(chǎn)品型號(hào): Hs 343.T
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡(jiǎn)單介紹

Hs 343.T細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。Hs 343.T細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長(zhǎng)密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


Hs 343.T細(xì)胞  的詳細(xì)介紹

Hs 343.T細(xì)胞

運(yùn)輸方式: 凍存運(yùn)輸

是否是腫瘤細(xì)胞: 1

物種來源: 人

ATCC Number: CRL-7245?

相關(guān)**: 腺癌

生長(zhǎng)狀態(tài): 貼壁生長(zhǎng)

年限: 47 years

數(shù)量: 大量

細(xì)胞形態(tài): 成纖維樣

器官來源: **

Designations: Hs 343.T

Hs 343.T細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast


Source: Organ: mammary gland; breast

Disease: adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Cytogenetic Analysis: modal number = 46; range = 45 to 46

Age: 47 years

Gender: Hs 343.T細(xì)胞female

Ethnicity: Caucasian

Comments: Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 2 to 3 days

Hs 343.T細(xì)胞Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium Hs 343.T細(xì)胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

滬公網(wǎng)安備 31011702004356號(hào)

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