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產(chǎn)品資料

Ramos (RA 1)細胞

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產(chǎn)品名稱: Ramos (RA 1)細胞
產(chǎn)品型號: Ramos (RA 1)
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關文檔

簡單介紹

Ramos (RA 1)細胞應如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術不當、操作室環(huán)境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術、清潔的環(huán)境、與品質(zhì)良好之細胞來源和培養(yǎng)基配制是減低污染之*好方法。Ramos (RA 1)細胞何時須更換培養(yǎng)基?視細胞生長密度而定,或遵照細胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


Ramos (RA 1)細胞  的詳細介紹

Ramos (RA 1)細胞

數(shù)量: 大量

運輸方式: 凍存運輸

生長狀態(tài): 懸浮生長

細胞形態(tài): **樣

**類型: IgM; lambda light chain

年限: 3 years

ATCC Number: CRL-1596?

相關**: 其他**

細胞類型: B**細胞

是否是腫瘤細胞: 1

物種來源: 人

Designations: Ramos (RA 1)

Ramos (RA 1)細胞Depositors: BC Giovanella

Isotype: IgM; lambda light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast


Source: Disease: Burkitt's lymphoma (American)

Cell Type: B lymphocyte;

Cellular Products: immunoglobulin (surface and secreted) [1093] [22747]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Receptors: interleukin 4 (IL-4); Ramos (RA 1)細胞low affinity IgE (CD23) [23468]

Tumorigenic: Yes

Antigen Expression: CD23 + [23468]

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 13,14

D16S539: 10,13

D5S818: 7,12

D7S820: 11

THO1: 7,9.3

TPOX: 8,9

vWA: 15,16

Age: 3 years

Gender: male

Ethnicity: Caucasian

Comments: The cells are negative for Epstein-Barr virus.

Both the parental line Ramos (RA 1)細胞(ATCC CRL-1596)and the derivative cell line Ramos.2G6.4C10 (ATCC CRL-1923) have about 1500 IL-4 binding sites per cell.

The cells are reported to secrete IgM (lambda light chain).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.

Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

derivative:ATCC CRL-1923

References: 1093: Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763

22747: Klein G, et al. Ramos (RA 1)細胞An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5: 319-334, 1975. PubMed: 181343

23389: Siegel JP, Mostowski HS. A bioassay for the measurement of human interleukin-4. J. Immunol. Methods 132: 287-295, 1990. PubMed: 1698879

23468: Rousset F, et al. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma. J. Immunol. 140: 2625-2632, 1988. PubMed: 2965726

26047: Nilsson K, et al. Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int. J. Cancer 19: 337-344, 1977. PubMed: 14896

32796: Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082

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