Daudi細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CCL-213?

相關(guān)**： **瘤

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

年限： 16 years

器官來源： 外周血

細(xì)胞類型： B**細(xì)胞

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來源： 人

細(xì)胞形態(tài)： **樣

**類型： IgM

Designations: Daudi

Daudi細(xì)胞Depositors: G Klein

Isotype: IgM

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: peripheral blood

Disease: Burkitt's lymphoma

Cell Type: B lymphoblast;

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Isolation: Isolation date: May, 1967

Applications: transfection host

Receptors: complement, expressed

Fc, expressed

Tumorigenic: Yes

DNA Profile (STR): Daudi細(xì)胞Amelogenin: X,Y

CSF1PO: 12

D13S317: 11,12

D16S539: 10,12

D5S818: 8,13

D7S820: 8,10

THO1: 6,7

TPOX: 8,11

vWA: 15,17

Cytogenetic Analysis: Male human karyotype with stemline number of 46. The karyotype is diploid in 66% of the cells and is stable within the stemline.

Isoenzymes: G6PD, B

Age: 16 years

Gender: male

Ethnicity: Black

Comments: The Daudi line was derived from a 16-year-old Black male with Burkitt's lymphoma by E. Klein and G. Klein in May, 1967. The cells are negative for beta-2-microglobulin. They are positive for EBNA, VCA and Surface immunoglobulin (sIg+). The line carries Epstein-Barr virus. The Daudi is a well characterized B lymphoblast cell line which has been employed extensively in studies of mechanisms of leukemogenesis.

Propagation: Daudi細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 to 5 X 10(5) viable cells/ml.

Interval: Maintain cell density between 3 X 10(5) and 2 to 3 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22550: Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523

23017: Klein E, et al. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res. 28: 1300-1310, 1968. PubMed: 4174339

26046: Huber C, et al. Surface receptors on human haematopoietic cell lines. Clin. Exp. Immunol. 25: 367-378, 1976. PubMed: 963908

26047: Nilsson K, et al. Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int. J. Cancer 19: 337-344, 1977. PubMed: 14896

28315: Gao Y, et al. Induction of an exceptionally high-level, nontranslated, Epstein-Barr virus-encoded polyadenylated transcript in the uurkitts lymphoma line Daudi. J. Virol. 71: 84-94, 1997. Daudi細(xì)胞PubMed: 8985326

32286: Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019

32830: Yamaguchi Y, et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed: 8943055

33091: Lewis JA, et al. Inhibition of mitochondrial function by interferon. J. Biol. Chem. 271: 13184-13190, 1996. PubMed: 8662694

33115: Montoya JG, et al. Human CD4+ and CD8+ T lymphocytes are both cytotoxic to Toxoplasma gondii-infected cells. Infect. Immun. 64: 176-181, 1996. PubMed: 8557337