AsPC-1細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

器官來(lái)源： 胰腺

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-1682?

相關(guān)**： 腺癌

年限： 62 years

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

Designations: AsPC-1

Depositors: MH Tan

AsPC-1細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology:

Source: Organ: pancreas

Disease: adenocarcinoma

Derived from metastatic site: ascites

Cellular Products: carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin

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Applications: transfection host

DNA Profile (STR):AsPC-1細(xì)胞 Amelogenin: X

CSF1PO: 10,13

D13S317: 9,12

D16S539: 11

D5S818: 12

D7S820: 12,13

THO1: 7,9.3

TPOX: 8,10

vWA: 17

Age: 62 years

Gender: female

Ethnicity: Caucasian

Comments: The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas.

Propagation: AsPC-1細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 2152: Chen WH, et al. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34, 1982. PubMed: 7182348

22552: Tan MH, et al. Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft. J. Natl. Cancer Inst. 67: 563-569, 1981. PubMed: 6944528

22737: Loor R, et al. AsPC-1細(xì)胞Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226

23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

26140: Tan MH, Chu TM. Characterization of the tumorigenic and metastatic properties of a human pancreatic tumor cell line (AsPC-1) implanted orthotopically into nude mice. Tumor Biol. 6: 89-98, 1985. PubMed: 4023565