SKO-007細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞類(lèi)型： B**細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
ATCC Number： CRL-8033-1?
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
**類(lèi)型： IgE; lambda light chain
Designations: SKO-007
Depositors: H Kaplan, Stanford University Board of Trustees
SKO-007細(xì)胞Isotype: IgE; lambda light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Cell Type: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: HLA A2, A3, B7, Bw60, Bw6, Cw3
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 12
SKO-007細(xì)胞D16S539: 10
D5S818: 11,12
D7S820: 11,12
THO1: 5,7
TPOX: 8
vWA: 17
Comments: This line was derived from the U266 myeloma cell line of Nilsson (Clin. Exp. Immunol. 7:477-489, 1970) by selecting for resistance to 8-azaguanine;the cells cells are HAT sensitive.
This is a batch of SKO-007 SKO-007細(xì)胞(formerly available as ATCC CRL-8033) that was cured at the ATCC of a mycoplasma contamination.
The J3 clone of SKO-007 is available also (ATCC CRL-8033-2).
Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.02 mg/ml 6-thioguanine, 1 mM sodium pyruvate and 2 mM L-glutamine, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
Subculturing: Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: parental cell line:ATCC CRL-8033
References: 1214: Olsson L, Kaplan HS. Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity. Proc. Natl. Acad. Sci. USA 77: 5429-5431, 1980. SKO-007細(xì)胞PubMed: 6159646
