SW948細(xì)胞

生長狀態(tài)： 貼壁生長

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CCL-237?

相關(guān)**： 大腸癌

器官來源： 結(jié)腸

細(xì)胞形態(tài)： 上皮樣

是否是腫瘤細(xì)胞： 1

物種來源： 人

年限： Dukes' type C, grade III

數(shù)量： 大量

Designations: SW948 [SW-948]

Depositors: A Leibovitz

SW948細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: colon

Tumor Stage: Dukes' type C, grade III

Disease: colorectal adenocarcinoma

Cellular Products: carcinoembryonic antigen (CEA) 7 ng/10 exp6 cells/10 days; colon specific antigen (CSAp) 750 units in 0.5 ml cell sonicate; keratin

Permits/Forms: SW948細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Oncogene: myc +; myb +; ras +; fos +; p53 +; sis -; abl -; ros -; src -

Antigen Expression: blood type O; Rh+

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 10,11

D16S539: 11,12

D5S818: 11

D7S820: 9,11

THO1: 6,9.3

TPOX: 8,11

vWA: 16,18

Isoenzymes: ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1-2

PGM3, 1-2

Age: 81 years

Gender: female

Ethnicity: SW948細(xì)胞Caucasian

Comments: The cells are positive for keratin by immunoperoxidase staining.

The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb and fos oncogenes.

N-myc and sis oncogene expression were not detected.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 100%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:15 is recommended

Medium Renewal: 1 to 2 times per week

Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37C. Add fresh medium, disperse cells and centrifuge to pellet the cells. Resuspend in fresh medium and dispense into new flasks.

References: 23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501

32265: Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. SW948細(xì)胞Cancer Res. 58: 109-113, 1998. PubMed: 9426066