RPMI 2650細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： CCL-30?

器官來(lái)源： 其他

相關(guān)**： 鱗狀細(xì)胞癌

年限： 52 years

細(xì)胞形態(tài)： 上皮樣

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

Designations: RPMI 2650

RPMI 2650細(xì)胞Depositors: GE Moore

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: nasal septum

Disease: squamous cell carcinoma

Derived from metastatic site: pleural effusion

Cellular Products: mucoid; keratin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. RPMI 2650細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: The cells are positive for keratin by immunoperoxidase staining.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 9,11

D13S317: 11,12

D16S539: 11,12

D5S818: 12,13

D7S820: 8,11

THO1: 6,8

TPOX: 8

vWA: 16,18

Isoenzymes: G6PD, B

Age: 52 years

Gender: male

Comments: The cells are positive for keratin by immunoperoxidase staining.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. RPMI 2650細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. 3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. 4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. 6. Incubate cultures at 37?C.NOTE: Cells attach in clusters. Cells will pile and the culture does not get 100% confluent.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Twice per week

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22218: Moore GE, Sandberg AA. Studies of a human tumor cell line with a diploid karyotype. Cancer 17: 170-175, 1964. PubMed: 14123677

25971: Moorhead PS. Human tumor cell line with a quasi-diploid karyotype (RPMI 2650). RPMI 2650細(xì)胞Exp. Cell Res. 39: 190-196, 1965. PubMed: 5831238