COLO 320HSR細(xì)胞
細(xì)胞形態(tài)： 其他
年限： Dukes' type C
生長(zhǎng)狀態(tài)： 貼壁，多細(xì)胞聚集
運(yùn)輸方式： 凍存運(yùn)輸
器官來(lái)源： 結(jié)腸
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
ATCC Number： CCL-220.1?
相關(guān)**： 大腸癌
數(shù)量： 大量
Designations: COLO 320HSR [COLO 320 HSR]
COLO 320HSR細(xì)胞Depositors: GE Moore
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: loosely adherent, multicell aggregates
Organism: Homo sapiens
Morphology: cells are rounded and refractile
Source: Organ: colon
Tumor Stage: Dukes' type C
Disease: colorectal adenocarcinoma
Cellular Products: serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone
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Tumorigenic: Yes
DNA Profile (STR): COLO 320HSR細(xì)胞Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11,12
D5S818: 12
D7S820: 9,12
THO1: 8,9
TPOX: 8,9
vWA: 15,18
Cytogenetic Analysis: marker chromosomes with homogeneously staining regions (HSR) were observed and the double minute (DM) chromosomes appeared in much lower frequency than the parental line COLO 320DM
Isoenzymes: ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 2
Age: 55 years
Gender:COLO 320HSR細(xì)胞 female
Ethnicity: Caucasian
Comments: CEA, negative.
CSAp, negative.
Colon antigen 3, positive.
The cells are weakly positive for keratins and vimentin.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a 0.25% trypsin, 0.53 mM EDTA solution. Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 3 to 4 days
Preservation: Freeze medium: FreezeMedium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:COLO 320HSR細(xì)胞ATCC 30-2020
parental cell line:ATCC CCL-220
References: 1045: Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117
22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
