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產(chǎn)品資料

NCI-H719細(xì)胞

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產(chǎn)品名稱: NCI-H719細(xì)胞
產(chǎn)品型號: NCI-H719
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

NCI-H719細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。NCI-H719細(xì)胞何時須更換培養(yǎng)基?視細(xì)胞生長密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


NCI-H719細(xì)胞  的詳細(xì)介紹

NCI-H719細(xì)胞

運(yùn)輸方式: 瓶裝運(yùn)輸

器官來源: 肺

ATCC Number: CRL-5837?

相關(guān)**: 小細(xì)胞肺癌

生長狀態(tài): 懸浮生長

年限: stage E

是否是腫瘤細(xì)胞: 1

物種來源: 人

數(shù)量: 大量

Designations: NCI-H719 [H719]

Depositors: AF Gazdar, JD Minna

NCI-H719細(xì)胞Biosafety Level: 1

Shipped: flask

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology:

Source: Organ: lung

Tumor Stage: stage E

Disease: carcinoma; classic small cell lung cancer

Derived from metastatic site: bone marrow

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NCI-H719細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

Applications: The line was established in April 1984.

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 12

D16S539: 9

D5S818: 13

D7S820: 10

THO1: 8,9

NCI-H719細(xì)胞TPOX: 8,11

vWA: 16,18

Age: 55 years

Gender: female

Ethnicity: Caucasian

Comments: The line was established in April 1984.

The patient was a smoker.

100 pack years.

Propagation: ATCC complete growth medium: HITES medium supplemented with 5% fetal bovine serum

The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium

0.005 mg/ml Insulin

0.01 mg/ml Transferrin

30nM Sodium selenite (final conc.)

10 nM Hydrocortisone (final conc.)

10 nM beta-estradiol (final conc.)

extra 2mM L-glutamine (for final conc. of 4.5 mM)

5% fetal bovine serum (final conc.)


Temperature: 37.0°C

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: NCI-H719細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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