HEC-1-B細(xì)胞

細(xì)胞形態(tài)： 上皮樣

數(shù)量： 大量

器官來源： **

是否是腫瘤細(xì)胞： 1

物種來源： 人

年限： 71 years

組織來源： endometrium

ATCC Number： HTB-113?

相關(guān)**： 腺癌

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

Designations: HEC-1-B

HEC-1-B細(xì)胞Depositors: H Kuramoto

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: uterus

Tissue: endometrium

Disease: adenocarcinoma

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Applications: transfection host (Roche Transfection Reagents)

Tumorigenic: Yes

Antigen Expression: Blood Type B; Rh+

DNA Profile (STR): HEC-1-B細(xì)胞Amelogenin: X

CSF1PO: 10,12

D13S317: 11,16

D16S539: 11,12

D5S818: 11,13

D7S820: 9,11

THO1: 6,7

TPOX: 8,11

vWA: 18

Cytogenetic Analysis: diploid to tetraploid with large submetacentric marker

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 2

PGM1, 1

PGM3, 1-2

Age: 71 years

Gender: female

Comments: HEC-1-B細(xì)胞This is a substrain of HEC-1-A (see ATCC HTB-112) isolated in 1968 by H. Kuramoto.

Unlike HEC-1-A, this substrain exhibited a stationary growth period between the 135th and 190th days in culture and appeared on recovery to be flattened and more pavement patterned than the parent line.

Futhermore, the predominant complement of chromosomes was double that observed for the parent line.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22449: Kuramoto H. Studies of the growth and cytogenetic properties of human endometrial adenocarcinoma in culture and its development into an established line. Acta Obstet. Gynaecol. Jpn. 19: 47-58, 1972. PubMed: 4678779

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

23069: Presta M, et al. HEC-1-B細(xì)胞Modulation of plasminogen activator activity in human endometrial adenocarcinoma cells by basic fibroblast growth factor and transforming growth factor beta. Cancer Res. 48: 6384-6389, 1988. PubMed: 3263185

23541: Kuramoto H, et al. Establishment of a cell line of human endometrial adenocarcinoma in vitro. Am. J. Obstet. Gynecol. 114: 1012-1019, 1972. PubMed: 4673779

32299: St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830

33112: Schramm N, et al. Vesicles containing Chlamydia trachomatis serovar L2 remain above pH 6 within HEC-1B cells. Infect. Immun. 64: 1208-1214, 1996. PubMed: 8606080