NCI-H747細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
器官來(lái)源： 盲腸
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
年限： 69 years
ATCC Number： CCL-252?
數(shù)量： 大量
相關(guān)**： 大腸癌
細(xì)胞形態(tài)： 上皮樣
Designations: NCI-H747 [H747]
Depositors: AF Gazdar
NCI-H747細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent, floating aggregates of round cells with some attached cells
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: cecum
Disease: colorectal adenocarcinoma
Cellular Products: carcinoembryonic antigen (CEA), 8 ng/ml per 10 exp6 cells per 10 days
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NCI-H747細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Antigen Expression: Blood type A; Rh+; CA19-9 antigen
Cytogenetic Analysis: modal number = 66; range = 63 to 75
This is a hypotriploid human cell line. The modal chromosome number is 66, occurring in 20% of cells. The rate of higher ploidies is 1.6%. Twelve marker chromosomes are common to all cells; these include single copy for i(1q), i(7p), i(10q); two copies for i(3q) and two to three copies for der(12)t(12;13)(q24,33;q12). The N12, N13, X and Y have single copy each. The presence of the Y was confirmed by Q-banding.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 69 years
Gender: male
Ethnicity: Caucasian
Comments: NCI-H747細(xì)胞This line was derived from a metastasis in a common duct node from a patient prior to therapy.
The cells are negative for Dopa decarboxylase and TAG-72 antigen but are positive for CEA and CA19-9 antigen.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% DMSO
Storage temperature: NCI-H747細(xì)胞liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249
