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產品資料

Hs 934.T細胞

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產品名稱: Hs 934.T細胞
產品型號: Hs 934.T
產品展商: HZbscience
產品文檔: 無相關文檔

簡單介紹

Hs 934.T細胞應如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術不當、操作室環(huán)境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術、清潔的環(huán)境、與品質良好之細胞來源和培養(yǎng)基配制是減低污染之*好方法。Hs 934.T細胞何時須更換培養(yǎng)基?視細胞生長密度而定,或遵照細胞株基本數據上之更換時間,按時更換培養(yǎng)基即可。


Hs 934.T細胞  的詳細介紹

Hs 934.T細胞

ATCC Number: CRL-7684?

相關**: 惡性黑色素瘤

數量: 大量

是否是腫瘤細胞: 1

物種來源: 人

組織來源: connective tissue

運輸方式: 凍存運輸

年限: 58 years

Designations: Hs 934.T

Biosafety Level: 1

Hs 934.T細胞Shipped: frozen

Medium & Serum: See Propagation

Organism: Homo sapiens

Morphology:

Source: Tissue: connective tissue

Disease: malignant melanoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Cytogenetic Analysis: Hs 934.T細胞modal number = 46; range = 45 to 47

Age: 58 years

Gender: female

Ethnicity: Caucasian

Comments: Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Please see the NBL Repository description.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Hs 934.T細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium Hs 934.T細胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

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