RL細(xì)胞

細(xì)胞類(lèi)型： B**細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

ATCC Number： CRL-2261?

相關(guān)**： 其他**

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

年限： 52 years

細(xì)胞形態(tài)： **樣

**類(lèi)型： IgM; lambda light chain

組織來(lái)源： ascites

Designations: RL

RL細(xì)胞Depositors: M Beckwith

Isotype: IgM; lambda light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Tissue: ascites

Disease: non-Hodgkin's lymphoma

Cell Type: B lymphoblast;

Cellular Products: immunoglobulin; surface IgM and IgD; secreted IgM (low levels)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. RL細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) -

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10

D13S317: 8,11

D16S539: 12

D5S818: 12

D7S820: 10,13

THO1: 9,10

TPOX: 9,11

vWA: 19,20

Cytogenetic Analysis: the cells possess a 14:18 translocation

Age: 52 years

Gender: male

Ethnicity: Caucasian

Comments: RL細(xì)胞RL is a human non-Hodgkin's lymphoma B cell line originated in 1983 by Walter J. Urba and Dan L. Longo.

Exposure of RL cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.

RL cells have been reported to be Epstein-Barr virus genome negative.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22555: Beckwith M, et al. RL細(xì)胞Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723