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產(chǎn)品資料

GCT細(xì)胞

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產(chǎn)品名稱: GCT細(xì)胞
產(chǎn)品型號: GCT
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

GCT細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。GCT細(xì)胞何時須更換培養(yǎng)基?視細(xì)胞生長密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


GCT細(xì)胞  的詳細(xì)介紹

GCT細(xì)胞

運輸方式: 凍存運輸

年限: 29 years

ATCC Number: TIB-223?

相關(guān)**: 其他**

生長狀態(tài): 貼壁生長

數(shù)量: 大量

是否是腫瘤細(xì)胞: 1

物種來源: 人

Designations: GCT [Giant Cell Tumor]

Depositors: J Brennan

GCT細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology:

Source: Disease: fibrous histiocytoma

Derived from metastatic site: lung

Cellular Products: colony stimulating activity (CSA); erythroid enhancing activity (EEA); prostaglandin E; plasminogen activator

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 11,12

D16S539: 9

GCT細(xì)胞D5S818: 13,15

D7S820: 11,12

THO1: 8,9.3

TPOX: 8,9

vWA: 16,18

Age: 29 years

Gender: male

Comments: The line produces CSA for human granulocyte precursors and EEA for erythroid precursor.

Medium conditioned by this line can be used as a source of prostaglandin E and plasminogen activator.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: GCT細(xì)胞Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, add fresh 0.25% trypsin for 2 to 3 minutes, add fresh medium, aspirate and dispense into new flasks.

References: 1181: Di Persio JF, et al. Human cell lines that elaborate colony-stimulating activity for the marrow cells of man and other species. Blood 51: 507-519, 1978. PubMed: 304748

1182: DiPersio JF, et al. The fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT. Blood 56: 717-727, 1980. PubMed: 6968234

1183: Abboud CN, et al. Hydrophobic adsorption chromatography of colony-stimulating activities and erythroid-enhancing activity from the human monocyte-like cell line, GCT. GCT細(xì)胞Blood 58: 1148-1154, 1981. PubMed: 6975643

3550: Lichtman MA, Brennan JK. Obtaining human cell lines that elaborate colony stimulating activity for marrow cells of man and other species and methods of preparing same. US Patent 4,135,975 dated Jan 23 1979

58064: Abboud CN, et al. Erythropoietic enhancing activity (EEA) secreted by the human cell line, GCT. J. Supramol. Struct. 13: 199-209, 1980. PubMed: 6972469

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