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產(chǎn)品資料

G-361細(xì)胞

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產(chǎn)品名稱: G-361細(xì)胞
產(chǎn)品型號: G-361
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

G-361細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。G-361細(xì)胞何時須更換培養(yǎng)基?視細(xì)胞生長密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


G-361細(xì)胞  的詳細(xì)介紹

G-361細(xì)胞

數(shù)量: 大量

器官來源: 皮膚

是否是腫瘤細(xì)胞: 1

物種來源: 人

ATCC Number: CRL-1424?

相關(guān)**: 惡性黑色素瘤

細(xì)胞形態(tài): 上皮樣

年限: 31 years *****

運(yùn)輸方式: 凍存運(yùn)輸

Designations: G-361

Depositors: PT Peebles

G-361細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Organism: Homo sapiens

Morphology: epithelial


Source: Organ: skin

Disease: malignant melanoma

Cellular Products: melanin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X

CSF1PO: 10

D13S317: 11

G-361細(xì)胞D16S539: 15

D5S818: 11

D7S820: 8,11

THO1: 7,9

TPOX: 8,10

vWA: 15,17

Isoenzymes: G6PD, B

Age: 31 years *****

Gender: male

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. G-361細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin - 0.53 mM EDTA for several minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 24 hours after seeding from a thawed ampule, then 2 to 3 times per week

References: 22209: . . Pediatr. G-361細(xì)胞Res. 12: 485, 1978.

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