DMS 153細胞

生長狀態(tài)： 單層、貼壁

運輸方式： 凍存運輸

器官來源： 肺

是否是腫瘤細胞： 1

物種來源： 人

ATCC Number： CRL-2064?

相關(guān)**： 小細胞肺癌

年限： 44 years

數(shù)量： 大量

Designations: DMS 153

Depositors: OS Pettengill, G Sorenson

Biosafety Level: 1

Shipped: DMS 153細胞frozen

Medium & Serum: See Propagation

Growth Properties: monolayer of adherent clusters

Organism: Homo sapiens

Morphology:

Source: Organ: lung

Disease: carcinoma; small cell lung cancer

Derived from metastatic site: liver

Cellular Products: adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; calcitonin gene related peptide (CGRP); oxytocin - neurophysin (OT-NP); 17 beta estradiol

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Restrictions: Note: DMS 153細胞These cells are distributed subject to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.) Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675. 3.) In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.

Receptors: bombesin; epidermal growth factor (EGF); complement (CR3)

Tumorigenic: Yes

Antigen Expression: Leu 7 +; My23 +; CD11b +

Age: 44 years

Gender: male

Ethnicity: Caucasian

Comments: The line was established from cells from liver tissue taken at autopsy of a patient with small cell carcinoma of the lung.

The patient had received prior therapy with cytoxan and methotrexate.

The cells express HLA class I and class II antigens.

Propagation: ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%

Subculturing: DMS 153細胞Medium Renewal: Twice per week

Remove the spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.

Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

Set up flasks at about 9 X 10 exp6 cells per 75 sq. cm. flask.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22793: Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. DMS 153細胞PubMed: 6266631