A431NS細(xì)胞
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
細(xì)胞形態(tài)： 上皮樣
器官來源： 皮膚
組織來源： epidermis
ATCC Number： CRL-2592?
相關(guān)**： 上皮細(xì)胞癌
是否是腫瘤細(xì)胞： 1
物種來源： 人
年限： 85 yrs
Designations: A431NS
Depositors: K Hirabayashi
A431NS細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens deposited as human
Morphology: epithelial
Source: Organ: skin
Tissue: epidermis
Disease: epidermoid carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 9,13
A431NS細(xì)胞D16S539: 12,14
D5S818: 12,13
D7S820: 10
THO1: 9
TPOX: 11
vWA: 15,17
Age: 85 yrs
Gender: female
Comments: A431NS was derived from the A431 cell line (ATCC CRL-1555) in 1997 by repeated subculturing to select cells that detached from the substrate easily.
The polyinosinic-polycytidylic acid/cationic liposome complex (LIC) inhibits cancer cell growth by the induction of apoptosis. [51526]
LIC has strong cytotoxic effects on the A431NS cell line but neither polyinosinic-polycytidylic acid alone nor cationic liposome alone have antiproliferation effects.
While cell sensitivity to drugs tend to be changeable, the A431NS cell line has a stable sensitivity to LIC. The cell line can be used to test the antiproliferation activity of LIC.
Propagation: A431NS細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium and rinse with PBS. Remove the PBS completely and add fresh 0.25% trypsin, 0.03% EDTA solution. Allow the flask to sit at room temperature (or 37C) until the cells detach. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperture
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-1555
References: 51526: A431NS細(xì)胞Hirabayashi K, et al. Inhibition of cancer cell growth by polyinosinic-polycytidylic acid/cationic liposome complex: a new biological activity. Cancer Res. 59: 4325-4333, 1999. PubMed: 10485480
