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產(chǎn)品資料

Hs 899(D)細(xì)胞

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產(chǎn)品名稱: Hs 899(D)細(xì)胞
產(chǎn)品型號: Hs 899(D)
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

Hs 899(D)細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。Hs 899(D)細(xì)胞何時須更換培養(yǎng)基?視細(xì)胞生長密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


Hs 899(D)細(xì)胞  的詳細(xì)介紹

Hs 899(D)細(xì)胞

年限: 20 years

器官來源: 骨

數(shù)量: 大量

ATCC Number: CRL-7645?

相關(guān)**: 骨肉瘤

是否是腫瘤細(xì)胞: 1

物種來源: 人

運輸方式: 瓶裝運輸

細(xì)胞形態(tài): 成纖維樣

Hs 899(D)細(xì)胞生長狀態(tài): 貼壁生長

Designations: Hs 899(D).T

Biosafety Level: 1

Shipped: flask

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast


Source: Organ: bone

Disease: osteosarcoma

Derived from metastatic site: lung

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Hs 899(D)細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 20 years

Gender: male

Ethnicity: Caucasian

Comments: See also ATCC CCL-211 and ATCC CRL-7517, 7622, 7623, 7624, 7631, 7632, 7633, 7634, 7635, 7642, 7643 and 7644 for lines derived from the same patient.

Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Please see the NBL Repository description.

Note: This item is distributed only within the 50 United States. It is not available for international distribution.

Propagation: Hs 899(D)細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:Hs 899(D)細(xì)胞ATCC 30-2020

滬公網(wǎng)安備 31011702004356號

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