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產(chǎn)品資料

VA-ES-BJ細(xì)胞

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產(chǎn)品名稱(chēng): VA-ES-BJ細(xì)胞
產(chǎn)品型號(hào): VA-ES-BJ
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無(wú)相關(guān)文檔

簡(jiǎn)單介紹

VA-ES-BJ細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類(lèi)可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o(wú)菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無(wú)菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來(lái)源和培養(yǎng)基配制是減低污染之*好方法。VA-ES-BJ細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長(zhǎng)密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


VA-ES-BJ細(xì)胞  的詳細(xì)介紹

VA-ES-BJ細(xì)胞

年限: 41 years

數(shù)量: 大量

ATCC Number: CRL-2138?

相關(guān)**: 其他**

細(xì)胞形態(tài): 上皮樣

生長(zhǎng)狀態(tài): 貼壁生長(zhǎng)

運(yùn)輸方式: 凍存運(yùn)輸

是否是腫瘤細(xì)胞: 1

物種來(lái)源: 人

Designations: VA-ES-BJ

VA-ES-BJ細(xì)胞Depositors: C Helson

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial


Source: Disease: epithelioid carcinoma

Derived from metastatic site: bone marrow

Cellular Products: granulocyte macrophage colony stimulating factor (GM-CSF)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Oncogene:VA-ES-BJ細(xì)胞 p53 +

Cytogenetic Analysis: near triploid karyotype with structural as well as numerical abnormalities; modal number = 65. Rearrangements of both chromosomes 1 and 9 with unknown materials were present., Five copies of chromosome 7 were seen in almost every cell, and there was no intact Y chromosome present. Cell to cell variation was due to numerical differences only, structural abnormalities were very consistent.

Age: 41 years

Gender: male

Ethnicity: Caucasian

Comments: VA-ES-BJ is an epithelioid sarcoma cell line derived in 1991 from a bone marrow metastasis removed from an ***** male with primary vertebral epithelioid carcinoma.

The cells are sensitive to hemin.

The cell are positive for immunohistochemical staining for the following: AE-1 and AE-3 cytokeratins, fas, epithelial membrane antigen (EMA), vimentin and p53.

VA-ES-BJ細(xì)胞Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 0.1 mM non-essential amino acids; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended

Medium Renewal: Twice per week

Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22334: . . Int. J. VA-ES-BJ細(xì)胞Oncol. 7: 51-56, 1995.

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